Alkaline Hydrolysis of Protein for Amino Acid Analysis
نویسندگان
چکیده
منابع مشابه
Amino Acid Sequence Analysis of Hemagglutinin Protein of H9N2 Isolated from Broilers in Tehran in 2007
Background and Aims: Since 1998, Iranian poultry industry has been affected by avian influenza (AI) virus, subtype H9N2. The association of high mortality and case report of H5N1 and H9N2 influenza virus in wild birds in recent years raised the suspicion of a possible new genetic modified AI virus. Methods: Partial nucleotide sequences and deduced amino acid of hemagglutinin (HA) genes of 4 H9...
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The importance of an accurate and convenient method of amino acid analysis for proteins and peptides is becoming increasingly obvious with the recent developments in biotechnology and protein engineering. The amino acid analyzer based on ion-exchange chromatography developed by Moore and Stein’,’ has remained the method of choice. The most commonly used conventional protocol of Hirs et aL3 util...
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15 صفحه اولAlkaline Hydrolysis of N-acetylolycine
A faster reaction of the cation is expected for the compounds with a free amino group in the acyl moiety. Such an increase was observed for Ala-Ala-pnitroan~lide b.ut not with Ala-p-nitroanilide. Fischer · et al. concluded that a larger inc-rease in k1 with the latter compound was compensated by a reductionin k2/ki. so that the observed k1k2/(k~1 + k2) term remains the same. This assumption of ...
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Previously we have shown that subcritical water may be used as an alternative to enzymatic digestion in the proteolysis of proteins for bottom-up proteomics. Subcritical water hydrolysis of proteins was shown to result in protein sequence coverages greater than or equal to that obtained following digestion with trypsin; however, the percentage of peptide spectral matches for the samples treated...
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ژورنال
عنوان ژورنال: Nippon kagaku zassi
سال: 1970
ISSN: 0369-5387,2185-0917
DOI: 10.1246/nikkashi1948.91.11_1068